Scientific Specifications
People were next taught to acquire a vaginal test that have an excellent Dacron-tipped swab and put they during the a tube that has a specimen-transport typical to own HPV assessment. The nursing assistant practitioner up coming did a standardized pelvic test. ples was in fact obtained that have Dacron-tipped swabs and you may listed in tubes that has had transportation medium to own HPV testing. To have Papanicolaou (Pap) assessment, a cytobrush was applied to gather cellular thing throughout the endocervix and you can a synthetic spatula was applied to gather tissue regarding the squamocolumnar junction and you will ectocervix.
Specimens was basically checked for HPV DNA by making use of a polymerase-chain-impulse (PCR) assay. 17 DNA are isolated through the use of the brand new QIAamp DNA bloodstream equipment (Qiagen) according to manufacturer’s process.
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